D9655

pBR322 Hae III Digest

for DNA electrophoresis

• Código UNSPSC: 12352202
• NACRES: NA.25

Propiedades

• Nivel de calidad: 100
• Formulario: liquid
• uso: 1000 uses (0.5 mg size); 200 uses (0.1 mg size)
• idoneidad: suitable for electrophoresis (DNA)
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Sigma′s pBR322 Hae III Digest contains 22 fragments from 8 - 587 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Aplicación

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

Componentes

Sigma′s pBR322 Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Otras notas

Contains 22 fragments, 8–587 bp

For optimal resolution, the recommended agarose gel concentration is 2.5.

Nota de preparación

Plasmid pBR322 DNA was digested with Hae III to completion, then purified by phenol extraction.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable