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Merck

D5652

Sigma-Aldrich

Disolución salina tamponada con fosfatos de Dulbecco

Modified, without calcium chloride and magnesium chloride, powder, suitable for cell culture

Sinónimos:

DPBS

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About This Item

Número MDL:
Código UNSPSC:
12352207
eCl@ss:
32129211
NACRES:
NA.25

Nivel de calidad

Formulario

powder

técnicas

cell culture | mammalian: suitable

pH

7.2-7.8

solubilidad

water: 9.6 g/L, clear

temp. de almacenamiento

2-8°C

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Descripción general

Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.

Aplicación

D-PBS is used to wash cells during preparation and serial transfer.
Dulbecco′s Phosphate Buffered Saline has been used:
  • as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloy
  • to dissolve Angelica gigas powder for microencapsulation
  • to wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assay
  • to wash amniotic membrane for cellular analyses

Acciones bioquímicas o fisiológicas

Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

Cantidad

Formulated to contain 9.6 grams of powder per liter of medium.

Otras notas

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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