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D1821

Sigma-Aldrich

DAF-FM

≥98% (HPLC)

Sinónimos:

4-Amino-5-methylamino-2′,7′-difluorescein, Diaminofluorescein-FM

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About This Item

Fórmula empírica (notación de Hill):
C21H14F2N2O5
Número de CAS:
254109-20-1
Peso molecular:
412.34
Número MDL:
MFCD29073049
Código UNSPSC:
12352204
ID de la sustancia en PubChem:
329798711
NACRES:
NA.77

Nivel de calidad

100

Ensayo

≥98% (HPLC)

Formulario

powder

condiciones de almacenamiento

desiccated

color

white to brown

solubilidad

DMSO: >1 mg/mL

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

cadena SMILES

CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(O)cc4Oc5cc(O)c(F)cc35

InChI

1S/C21H14F2N2O5/c1-25-13-3-2-8-18(19(13)24)20(28)30-21(8)9-4-11(22)14(26)6-16(9)29-17-7-15(27)12(23)5-10(17)21/h2-7,25-27H,24H2,1H3

Clave InChI

DIJCILWNOLHJCG-UHFFFAOYSA-N

Aplicación

DAF-FM has been used in the visualization of nitric oxide (NO) in root sections of wheat seedlings. It has also been used to label neural crest (NC) cells.

Acciones bioquímicas o fisiológicas

DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO).
DAF-FM is a highly sensitive, photo-stable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM is more sensitive to nitric oxide (NO) compared with DAF-2. DAF-FM is more photo-stable and less pH sensitive than DAF-2. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Precaución

hygroscopic

Nota de preparación

Color variation within the range from white to brown does not change performance.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
047M4748V
035M4796V
071M4702V
030M4726
109K4712

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Judy Choi et al.
Environmental health perspectives, 118(5), 589-595 (2010-05-05)
Silica nanoparticles (SiNPs) are being formulated for cellular imaging and for nonviral gene delivery in the central nervous system (CNS), but it is unclear what potential effects SiNPs can elicit once they enter the CNS. As the resident macrophages of
Kunal Bhattacharya et al.
Advanced healthcare materials, 7(6), e1700916-e1700916 (2018-01-16)
Polyethylene glycol (PEG)-modified carbon nanotubes have been successfully employed for intra-articular delivery in mice without systemic or local toxicity. However, the fate of the delivery system itself remains to be understood. In this study 2 kDa PEG-modified single-walled carbon nanotubes
Fernando Toshio Ogata et al.
PloS one, 8(12), e84588-e84588 (2014-01-01)
Thioredoxin (TRX-1) is a multifunctional protein that controls the redox status of other proteins. TRX-1 can be found in the extracellular milieu, cytoplasm and nucleus, and it has distinct functions in each environment. Previously, we studied the intracellular localization of
Visualization of Nitric Oxide, Measurement of Nitrosothiols Content, Activity of NOS and NR in Wheat Seedlings
Adavi SB, et al.
Bio-protocol, 9(20) (2019)
Phillip Roder et al.
Scientific reports, 8(1), 5892-5892 (2018-04-14)
Local manipulation of complex tissues at the single-cell level is challenging and requires excellent sealing between the specimen and the micromanipulation device. Here, biological applications for a recently developed loading technique for a force- and pressure-controlled fluidic force microscope micropipette
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