CS0004

Nuclease micrococcal from Staphylococcus aureus

With reaction buffer and BSA solution

• Sinónimos: Nuclease micrococcal from Staphylococcus aureus, Endonuclease micrococcal, MNase, Micrococcal endonuclease
• Número de CAS: 9013-53-0
• Comisión internacional de enzimas: 3.1.31.1 (BRENDA, IUBMB)
• Número MDL: MFCD00131731
• Código UNSPSC: 12352202
• NACRES: NA.77

Propiedades

• origen biológico: Staphylococcus aureus
• formulario: liquid
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes

Aplicación

• Chromatin Immuno-Percipitation (ChIP) analysis
• Nucleosome preparation
• Removal of nucleic acids present in protein preparation
• Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation
• RNA sequencing and transcriptomic profiling

Acciones bioquímicas o fisiológicas

Hydrolyzes 5′-phosphodiester bonds of DNA.

Otras notas

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids