CN304-05
Canine Aortic Endothelial Cells: CnAOEC (Cryovial)
Sinónimos:
Endothelial cell line
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200 MG
560,32 $CA
560,32 $CA
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200 MG
560,32 $CA
About This Item
Código UNSPSC:
41106514
NACRES:
NA.81
560,32 $CA
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
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origen biológico
canine aorta
envase
pkg of 500,000 cells
fabricante / nombre comercial
Cell Applications, Inc
modo de crecimiento
Adherent
cariotipo
2n = 78
morfología
Endothelial
técnicas
cell culture | mammalian: suitable
enfermedades relevantes
diabetes; cardiovascular diseases
Condiciones de envío
dry ice
temp. de almacenamiento
−196°C
1 of 4
Este artículo | MAK133 | MAK113 | CS0012 |
|---|---|---|---|
| detection method chemiluminescent | detection method chemiluminescent | detection method colorimetric | detection method - |
| usage sufficient for 100 tests (bioluminescent) | usage sufficient for 100 assays (bioluminescent) | usage sufficient for 200 colorimetric tests | usage - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| application(s) pharmaceutical | application(s) - | application(s) - | application(s) - |
| relevant disease(s) cancer | relevant disease(s) hematological disorder; cancer | relevant disease(s) cancer | relevant disease(s) - |
Descripción general
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CnAOEC provide a useful model to study cardiovascular diseases and test potential therapeutic agents, not only important from a veterinary perspective, but also having implications for human health. In a study aiming to adapt an immunomagnetic isolation protocol used in human patients to isolate circulating endothelial cells in dogs, CnAOEC cells were shown to be positive for von Willebrand factor, CD146 and stained with Ulex europaeus agglutinin 1 (Wills, 2009). To investigate the mechanism of N-terminal portion of pro C-type natriuretic peptide (NT-pCNP), a sepsis biomarker in humans and dogs, CnAOEC were treated with a variety of agents and it was found that lipopolysaccharide, TNF-α and IL-1β, but not IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A, stimulated NT-pCNP production (Osterbur, 2012, 2013). CnAOEC were also used to demonstrate vasoprotective activity of pomegranate and soy isoflavons in order to prevent endothelial disfunction in dogs which commonly leads to cardiovascular disease (Baumgartner-Parzer, 2012). Finally, CnAOEC were utilized as a control to compare the growth of normal canine endothelial cells and aggressive canine hemangiosarcoma cells in a study investigating signaling pathways underlying hemangiosarcoma oncogenesis (Murai, 2012), and as a VEGFR2-positive control in a study aimed at understanding the reasons for reduced numbers of circulating progenitor cells in cardiovascular disease (Boilson, 2010).
CnAOEC provide a useful model to study cardiovascular diseases and test potential therapeutic agents, not only important from a veterinary perspective, but also having implications for human health. In a study aiming to adapt an immunomagnetic isolation protocol used in human patients to isolate circulating endothelial cells in dogs, CnAOEC cells were shown to be positive for von Willebrand factor, CD146 and stained with Ulex europaeus agglutinin 1 (Wills, 2009). To investigate the mechanism of N-terminal portion of pro C-type natriuretic peptide (NT-pCNP), a sepsis biomarker in humans and dogs, CnAOEC were treated with a variety of agents and it was found that lipopolysaccharide, TNF-α and IL-1β, but not IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A, stimulated NT-pCNP production (Osterbur, 2012, 2013). CnAOEC were also used to demonstrate vasoprotective activity of pomegranate and soy isoflavons in order to prevent endothelial disfunction in dogs which commonly leads to cardiovascular disease (Baumgartner-Parzer, 2012). Finally, CnAOEC were utilized as a control to compare the growth of normal canine endothelial cells and aggressive canine hemangiosarcoma cells in a study investigating signaling pathways underlying hemangiosarcoma oncogenesis (Murai, 2012), and as a VEGFR2-positive control in a study aimed at understanding the reasons for reduced numbers of circulating progenitor cells in cardiovascular disease (Boilson, 2010).
Origen línea celular
Aorta
Aplicación
cardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testing
Componentes
Canine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO
Nota de preparación
- 2nd passage, >500,000 cells in Canine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO
- Can be cultured at least 16 doublings
Rutina de subcultivo
Please refer to the CnAOEC Culture Protocol.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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Susan M Daly et al.
Langmuir : the ACS journal of surfaces and colloids, 22(3), 1215-1222 (2006-01-25)
We formed monolayers and black lipid membranes (BLMs) of photopolymerizable lipids mixed with the channel-forming protein gramicidin A to evaluate their miscibility and the potential for improved stability of the BLM scaffold through polymerization. Analyses of surface pressure vs area
Grzegorz Godlewski et al.
The Journal of pharmacology and experimental therapeutics, 328(1), 351-361 (2008-10-17)
The novel endocannabinoid-like lipid N-arachidonoyl L-serine (ARA-S) causes vasodilation through both endothelium-dependent and -independent mechanisms. We have analyzed the vasorelaxant effect of ARA-S in isolated vascular preparations and its effects on Ca(2+)-activated K(+) currents in human embryonic kidney cells stably
S Punnamaraju et al.
Langmuir : the ACS journal of surfaces and colloids, 28(20), 7657-7664 (2012-05-03)
A combination of nonpolymerizable phospholipids (DPPC or DPhPC) and a smaller amount of cross-linking photopolymerizable phospholipids (23:2 DiynePC) is incorporated in an unsupported artificial lipid bilayer formed using the droplet interface bilayer (DIB) approach. The DIB is formed by contacting
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