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CN304-05

Sigma-Aldrich

Canine Aortic Endothelial Cells: CnAOEC (Cryovial)

Sinónimos:

Endothelial cell line

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200 MG
560,32 $CA

560,32 $CA


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200 MG
560,32 $CA

About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

560,32 $CA


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origen biológico

canine aorta

envase

pkg of 500,000 cells

fabricante / nombre comercial

Cell Applications, Inc

modo de crecimiento

Adherent

cariotipo

2n = 78

morfología

Endothelial

técnicas

cell culture | mammalian: suitable

enfermedades relevantes

diabetes; cardiovascular diseases

Condiciones de envío

dry ice

temp. de almacenamiento

−196°C

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Este artículo
MAK133MAK113CS0012
ADP Assay Kit sufficient for 100 assays (bioluminescent)

MAK133

ADP Assay Kit

detection method

chemiluminescent

detection method

chemiluminescent

detection method

colorimetric

detection method

-

usage

sufficient for 100 tests (bioluminescent)

usage

sufficient for 100 assays (bioluminescent)

usage

sufficient for 200 colorimetric tests

usage

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

application(s)

pharmaceutical

application(s)

-

application(s)

-

application(s)

-

relevant disease(s)

cancer

relevant disease(s)

hematological disorder; cancer

relevant disease(s)

cancer

relevant disease(s)

-

Descripción general

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CnAOEC provide a useful model to study cardiovascular diseases and test potential therapeutic agents, not only important from a veterinary perspective, but also having implications for human health. In a study aiming to adapt an immunomagnetic isolation protocol used in human patients to isolate circulating endothelial cells in dogs, CnAOEC cells were shown to be positive for von Willebrand factor, CD146 and stained with Ulex europaeus agglutinin 1 (Wills, 2009). To investigate the mechanism of N-terminal portion of pro C-type natriuretic peptide (NT-pCNP), a sepsis biomarker in humans and dogs, CnAOEC were treated with a variety of agents and it was found that lipopolysaccharide, TNF-α and IL-1β, but not IL-6, IL-10, IL-21, CXCL-8, IFN-γ, VEGF-A, stimulated NT-pCNP production (Osterbur, 2012, 2013). CnAOEC were also used to demonstrate vasoprotective activity of pomegranate and soy isoflavons in order to prevent endothelial disfunction in dogs which commonly leads to cardiovascular disease (Baumgartner-Parzer, 2012). Finally, CnAOEC were utilized as a control to compare the growth of normal canine endothelial cells and aggressive canine hemangiosarcoma cells in a study investigating signaling pathways underlying hemangiosarcoma oncogenesis (Murai, 2012), and as a VEGFR2-positive control in a study aimed at understanding the reasons for reduced numbers of circulating progenitor cells in cardiovascular disease (Boilson, 2010).

Origen línea celular

Aorta

Aplicación

cardiovascular function, studies on immune system and graft rejection, development of 3D endothelialized engineered tissues, drug discovery, stent-graft compatibility testing

Componentes

Canine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO

Nota de preparación

  • 2nd passage, >500,000 cells in Canine Endothelial Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 16 doublings

Rutina de subcultivo

Please refer to the CnAOEC Culture Protocol.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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