CMC0019
OverExpress™ C43(DE3) Chemically Competent Cells
Escherichia coli, rod shaped
Sinónimos:
C43 strain
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About This Item
Código UNSPSC:
41106202
NACRES:
NA.85
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Nombre del producto
OverExpress™ C43(DE3) Chemically Competent Cells, for the highest protein expression
origen biológico
Escherichia coli
grado
for molecular biology
modo de crecimiento
adherent or suspension
morfología
rod shaped
técnicas
microbiological culture: suitable
transformación celular
competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg
Condiciones de envío
dry ice
temp. de almacenamiento
−70°C
Categorías relacionadas
Descripción general
OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress™ C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Aplicación
OverExpress™ C43(DE3) Chemically Competent Cells have been used for transforming the toll/interleukin receptor (TIR) domain constructs of sterile alpha and TIR motif–containing protein 1 (SARM1).
Características y beneficios
- Express genes cloned into any T7 vector with these BL21(DE3) derivatives
- Effective in expressing toxic & membrane proteins
- Cited in over 350 research articles
Componentes
- OverExpress C43(DE3) chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Información legal
OverExpress is a trademark of Imaxio, S.A.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
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Artículos
Bacterial transformation is a process of horizontal gene transfer by which some bacteria take up foreign genetic material (naked DNA) from the environment. Bacteria that can take up free, extracellular genetic material are known as competent cells.
Protocolos
Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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