CLLS1214

MCF10A CELLS HER2 (-/-) EXON 2

human female maMilliporeary glands (Source Disease: Fibrocystic Disease)

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• Nombre del producto: MCF10A CELLS HER2 (-/-) EXON 2,
• origen biológico: human female mammary glands (Source Disease: Fibrocystic Disease)
• Nivel de calidad: 200
• Nº de acceso OMIM: 164870
• temp. de almacenamiento: −196°C
• Información sobre el gen: human ... ERBB2(2064) , HER2(2064)

Descripción

Descripción general

MCF10A CELLS HER2 (-/-) EXON 2 are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN modification.

This cell line was derived from ATCC Catalog No. CRL-10317.

Aplicación

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:8

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat. No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat. No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat. No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat. No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat. No. H6909, to a final concentration of 0.5 ug/mL.

Cell freezing medium - DMSO 1X, Cat. No. C6164.

Características y beneficios

These MCF10A cells are adherent with a doubling time of approx. 20 hours

Zinc finger nuclease (ZFN) modification on chromosome 17q21.1

Calidad

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Solo componentes del kit

• MCF10A CELLS HER2 (-/-) EXON 2 1 vial

Los componentes del kit también están disponibles por separado

• MCF10A CONTROL CELLS 1 vial

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable