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CHOGS

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CHOZN® ZFN-Modified CHO Cell Line

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.75

descripción

CHOZN® GS-/- ZFN-modified CHO cell line
glutamine synthetase knockout cell line

solubilidad

DMSO: 5 mg/mL, clear

Condiciones de envío

liquid nitrogen

temp. de almacenamiento

−196°C

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Descripción general

CHOZN cell lines were created utilizing Sigma′s proprietary CompoZr Zinc Finger Nuclease (ZFN) technology to create genetic modifications in parental CHO-K1 cells. Cells are banked in chemically defined, animal component free EX-CELL CD CHO Fusion media
ZFNs are a class of engineered DNA-binding proteins and causes a double-strand break. This double-strand break is necessary for site-specific mutagenesis mediated DNA repair. It facilitates targeted genome editing by binding to a user-specified locus. Glutamine synthetase (GS) is one of the most commonly used selectable markers in the biopharmaceutical industry. Glutamine is an essential amino acid for cellular growth. The GS enzyme is responsible for the conversion of glutamate into glutamine. Without this enzymatic activity, cells can no longer synthesize glutamine endogenously and if glutamine is not supplemented in the culture media, the cells will die.

Origen línea celular

CHOZN cell lines are all subclones of the parental CHO-K1 cell line originated by Puck in 1957.

Envase

This product is shipped in liquid nitrogen dry vapor shippers.
Liquid nitrogen dry vapor shipper fee (up to a maximum of $125) and freight not included in price of product.
Liquid nitrogen dry vapor shipper is not disposable and should be returned after receipt. A $450 liquid nitrogen dry vapor shipper fee will be charged if not returned within 1 week of delivery.
Learn more about liquid nitrogen dry vapor shipper

Forma física

CHOZN cell lines are provided to customers in vials containing >7.5e6 cells/mL

Información legal

All CHOZN cell lines are sold under license from Sigma Aldrich. A copy of the research license is provided with purchase of the CHOZN cell lines.
CHOZN is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
2113-53667
051M0924

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The metabolic profile of tumors depends on both the responsible genetic lesion and tissue type.
Yuneva M O, et al.
Cell Metabolism, 15(2), 157-170 (2012)
Cytotoxic effects during knock out of multiple porcine endogenous retrovirus (PERV) sequences in the pig genome by zinc finger nucleases (ZFN).
Semaan M, et al.
PLoS ONE, 10(4), e0122059-e0122059 (2015)
Q's next: the diverse functions of glutamine in metabolism, cell biology and cancer.
DeBerardinis R J and Cheng T
Oncogene, 29(3), 313-313 (2010)

Artículos

Impact of DMSO And Freezing Technique in Developing a High Cell Density Cryopreservation Process for Upstream Bioprocessing

Understand how considerations such as DMSO concentrations and freezing techniques are key in the successful implementation of high cell density cryopreservation (HCDC).

Using Microarray Technology to Select Housekeeping Genes in Chinese Hamster Ovary Cells

In the present study, we have identifi ed species-specifi c housekeeping genes (HKGs) for Chinese Hamster Ovary (CHO) cells using data from microarray gene expression profiling.

Signal Peptide Optimization: Effect On Recombinant Monoclonal IgG Productivity, Product Quality And Antigen-Binding Affinity

A signal peptide is a 5-30 amino acid (aa) peptide present at the N-terminus of secretory proteins.

Selection Process Development in a GS Knock-Out CHO Host Cell Line: The Effects of MSX Addition on Clones Generated in an MSX-Free Process

The Glutamine Synthetase (GS) expression system does not typically require multiple rounds of amplification to isolate high-producing clones (Brown, 1992).

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