LB185

98062318, human blood, Lymphoblastoid

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: LB185, 98062318
• origen biológico: human blood
• envase: tube of 5 μg 98062318-DNA-5UG; pkg of vial of cells 98062318-1VL
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human lymphoblastoid, normal,EBV-transformed

Descripción línea celular

The lymphocyte cell line LB185 has been established using mononuclear cells from peripheral blood of a 56 year old normal male. T cells from the same sample have been used as controls for cellular radiosensitivity studies. Cells have been subsequently transformed with EBV. Fibroblasts have been derived from the same individual and were designated 153BR (Sigma Catalogue number. 90011808). This has been confirmed by STR profiling at ECACC. Cells are lymphhoblastoid with floating aggregates present.

Aplicación

Mutation studies

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18

Medio de cultivo

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Rutina de subcultivo

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml; 5% CO₂, 37°C. Cells form floating aggregates.

Otras notas

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