MFE-296

98031101, human endometrium, Epithelial

• Sinónimos: MFE296 Cells
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: MFE-296, 98031101
• origen biológico: human endometrium
• modo de crecimiento: Adherent
• cariotipo: Near Tetraploid
• morfología: Epithelial
• productos: Not specified
• receptores: Androgen receptor
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human caucasian endometrial adenocarcinoma

Descripción línea celular

MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.

Aplicación

Tumour biology studies, receptor analysis

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18

Medio de cultivo

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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