EMT6/MTXR

96042322, mouse unknown/unspecified, Epithelial-like

• Código UNSPSC: 41106514

Propiedades

• product name: EMT6/MTXR, 96042322
• origen biológico: mouse unknown/unspecified
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse mammary, drug-resistant

Descripción línea celular

The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344) by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.

Aplicación

Drug-resistance studies

Medio de cultivo

MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml methotrexate + 15% Foetal Bovine Serum (FBS).. Resuscitate in drug-free medium; add drug once growth is fully established

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA;5% CO₂; 37ºC

Otras notas

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