XrS6-hamKu80

96012301, hamster ovary, Not specified

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: XrS6-hamKu80, 96012301
• origen biológico: hamster ovary
• envase: tube of 5 μg 96012301-DNA-5UG; pkg of vial of cells 96012301-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Not specified
• productos: Ku80
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Hamster Chinese ovary, transformed with hamster Ku80 cDNA

Descripción línea celular

The cell line Xrs6-hamKu80 was derived from a radiosensitive CHO-K1 mutant transfected with hamster Ku80 cDNA. Ku80, a subunit of the Ku protein and component of the DNA-dependent protein kinase, is a gene product defective in radiosensitive CHO cells, so called xrs mutants. XRC5, the gene encoding Ku80, belongs to the ionisation radiation group affected in DNA repair defects. Mutants transfected with hamster Ku80 obtained full restoration of gamma-ray resistance, DNA end-binding activity to parent levels and expression of Ku80 and Ku70 proteins.

Aplicación

Study of DNA repair mechanisms

Medio de cultivo

Ham′s F12 + 2mM Glutamine + 300μg/ml G418 + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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