M15

95102517, mouse kidney, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: M15, 95102517
• origen biológico: mouse kidney
• modo de crecimiento: Adherent
• cariotipo: Hyperdiploid
• morfología: Epithelial
• productos: WT1 (expressed gene)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse mesonephric epithelium, polyoma virus large T transformed

Descripción línea celular

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

Aplicación

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

Otras notas

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