BF-11

95050413, Reactivity: Anti-Erythropoietin, human

• Código UNSPSC: 41106514

Propiedades

• origen biológico: Rat x Mouse Hybridoma
• envase: tube of 5 μg 95050413-DNA-5UG; pkg of vial of cells 95050413-1VL
• ImmunoDon: Rat AC1 spleen cells
• Mieloma: Sp2/0 Ag-14
• Reactividad: Anti-Erythropoietin, human
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat ACI spleen x Mouse Sp2/0 Ag-14

Descripción línea celular

The BF-11 hybridoma cell line secretes a rat monoclonal antibody named Epoclonalan that reacts with human erythropoietin. Spleen cells from ACI strain rats that had been immunised with human urinary erythropoietin have been fused with Sp2/0 Ag-14 to produce this cell line. The α, β and asialo forms of human erythropoietin are bound by the antibody to the same extent.

Inmunógeno

Human erythropoietin

Aplicación

Immunological studies, isolation of erythropoietin by immuno-affinity methods.

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Medio de cultivo

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

Otras notas

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