AFRC 17D1

94120825, Application: Fusion partner for hybridoma production

• Código UNSPSC: 41106514

Propiedades

• origen biológico: Horse x Mouse/Horse Hybridoma
• ImmunoDon: Horse peripheral lymphocytes
• Mieloma: NS0
• Reactividad: Application: Fusion partner for hybridoma production
• técnicas: cell culture | mammalian: suitable
• isotipo: Not specified
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Horse peripheral blood lymphocytes x Mouse NS0

Descripción línea celular

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

Inmunógeno

Influenza A equine 2 virus

Aplicación

Fusion partner for hybridoma production

Medio de cultivo

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Otras notas

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