HF1-5

94101909, rat liver, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: HF1-5, 94101909
• origen biológico: rat liver
• modo de crecimiento: Adherent
• cariotipo: 100 chromosomes
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat hepatoma Hybrid [H5xFao]

Descripción línea celular

HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.

Aplicación

Gene analysis and liver function expression.

Medio de cultivo

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.