C2-Rev 7

94101907, rat liver, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: C2-Rev 7, 94101907
• origen biológico: rat liver
• modo de crecimiento: Adherent
• cariotipo: Hyperdiploid, 51 chromosomes
• morfología: Epithelial
• productos: Albumin and hepatocyte markers
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat hepatoma [HGPRT-]

Descripción línea celular

C2-Rev 7 is a revertant of the C2 variants, selected by virtue of its capacity to grow in glucose free medium (containing dialysed serum) where survival requires activity of the liver-specific gluconeogenic pathway. Expression of the entire set of liver specific functions is restored. Albumin and hepatocyte markers present.

Aplicación

Gene expression studies.

Medio de cultivo

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split semi-confluent cultures (70%) 1:10 i.e. seeding at 9x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density is reached at 15x1,000,000 cells/10cm² dish.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.