H5

94101905, rat liver, Fusiform

• Código UNSPSC: 41106514

Propiedades

• product name: H5, 94101905
• origen biológico: rat liver
• modo de crecimiento: Adherent
• cariotipo: Modal no. 52 (50-55)
• morfología: Fusiform
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat hepatoma

Descripción línea celular

H5 is a subclone of H4-II-E-C3 (Sigma Catalogue number. 85061112) which originated from the Reuber H-35 hepatoma induced by chemical carcinogenesis in an A x C rat. The dedifferentiated H5 cells express high levels of glucose-6-phosphate dehydrogenase and exhibit a population doubling time of 12 hours. Differentiated variants are H4IIEC3 (85061112), Fao and others.

Aplicación

Toxicity studies; negative control for differentiated hepatoma

Medio de cultivo

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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