SK-N-DZ

94092305

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human nerve (bone marrow metastasis)
• modo de crecimiento: Adherent
• cariotipo: 44XX, monosomic for 10, 11, 13, 14, 19. Both copies of chromosome 2 missing in each cell.
• morfología: Neuronal
• productos: p53, moderate MDR-1 expression
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human neuroblastoma

Descripción línea celular

Spontaneously transformed cells from a female patient with neuroblastoma. The tumour was derived from a bone marrow metastasis and is tumourigenic in nude mice. Used in the study of MIBG uptake in the diagnosis and treatment of neuroblastoma. MIBG is a derivative of the antihypertensive agent guanethidine and is used as a radiopharmaceutical. SK-N-DZ is characterised by low saturable kinetics and predominant linear pattern of uptake of MIBG, with a poor ability to maintain high intracellular levels of the drug.

Aplicación

Tumour studies. MRD-1 resistance studies. Extremely sensitive to cis-retinoic acid.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,11
D5S818: 12
D7S820: 12,13
THO1: 6,9
TPOX: 8
vWA: 16,18

Medio de cultivo

DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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