SK-N-F1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94092304, human nerve (bone marrow metastasis), Epithelial-like

• Código UNSPSC: 41106514

Propiedades

• origen biológico: human nerve (bone marrow metastasis)
• envase: tube of 5 μg 94092304-DNA-5UG; pkg of vial of cells 94092304-1VL
• modo de crecimiento: Adherent/aggregates in suspension
• cariotipo: Aneuploid
• morfología: Epithelial-like
• productos: Not specified
• receptores: Transferrin receptor
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human neuroblastoma

Descripción línea celular

SK-N-F1 has been obtained from a bone marrow metastasis of a male patient with neuroblastoma. The poorly differentiated and slow growing cells secrete different neuroblastoma specific peptides and synthesise choline acetyltransferase. Reactivity of anti-leukaemia-associated and anti-foetal brain-associated monoclonal antibodies could be shown. Epithelial-like and aggregate-forming populations.

Aplicación

Carcinogenesis studies, cytotoxicity assays.

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,14
D16S539: 11,12
D5S818: 12,13
D7S820: 8,9
THO1: 6,9.3
TPOX: 8,9
vWA: 16,17

Medio de cultivo

DMEM + 4mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/ml using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Clumped cells have tendency to float.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.