KT3 human cell line

IHW Number: 9107, 94070705

• Sinónimos: LKT3
• Código UNSPSC: 41106514

Propiedades

• origen biológico: human blood
• etnicidad: Japanese
• género: male
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice

Descripción

Descripción general

Homozygous: Yes

Descripción línea celular

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Perfil del ADN

A: 2402
Cw: 102
B: 5401
HLA DRA: 101
HLA DRB1: 405
HLA DRB4: 101
HLA DQA1: 3
HLA DQB1: 401
HLA DPA1: 2022
HLA DPB1: 501

Perfil serológico

HLA A: 24
HLA A ief: 24.1
HLA C: 1
HLA B: 54
HLA B ief: 54.1
Bw4/bw6: 6
HLA Dw: 15
HLA DR: 4
HLA DR53: 53
HLA DQ: 4
HLA DP: 5

Medio de cultivo

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rutina de subcultivo

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cláusula de descargo de responsabilidad

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.