B16 melanoma 4A5 Cell Line from mouse

94042254, mouse skin, Fibroblast-like

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: B16 melanoma 4A5 Cell Line from mouse, Mouse melanoma, producing melanin, 94042254
• origen biológico: mouse skin
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast-like
• productos: Melanin
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse melanoma, melanin producing

Descripción línea celular

Derived from a melanoma from the skin of a C57BL/6 strain mouse, showing fibroblast-like characteristics, which produces melanin. Cells may lose ability to produce pigmentation in long term culture.

Aplicación

B16 melanoma 4A5 cell line has been used to study the spectral and biological characteristics of copper (II) mesoporphyrinic complexes. It has also been used to evaluate the cytotoxicity of JaZ-30, a matrix metalloproteinase-2 (MMP-2) inhibitor.

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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