DSL6A/C1

94022431, rat pancreas, Polygonal

• Código UNSPSC: 41106514

Propiedades

• product name: DSL6A/C1, 94022431
• origen biológico: rat pancreas
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Polygonal
• productos: Expresses CFTR protein
• receptores: Lacks CCK-A receptors
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat pancreatic carcinoma

Descripción línea celular

DSL6A/C1 were derived from a transplantable acinar cell carcinoma (DSL-6) that had been established from a primary carcinoma of the pancreas in an azaserine treated Lewis rat. The expression of the ductal cell marker OC.2 was shown with a sub-population expressing ductal marker OV-6, CK-7 and CK-19. DSL6A/C1 cells are reported to be tumorigenic in Lewis rats and produce solid tumours composed of duct like structures surrounded by dense fibrous tissue. The cell line lost structural and immunohistochemical acinar cell markers while acquiring duct cell markers during culture and re-grafting.

Aplicación

Study of pancreatic carcinomas, differentiation of acinar/ductal cells.

Medio de cultivo

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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