EZZ (TOU II-4)

93093003, human blood, Lymphoblast

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: EZZ (TOU II-4), 93093003
• origen biológico: human blood
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human B cell lymphocyte, EBV transformed

Descripción línea celular

Peripheral blood lymphocytes from a male Tunisian, immortalised by EBV transformation. The cell line contains the following deletions, at the homozygous state, in the human immunoglobulin heavy chain constant region locus (IGH chromosome 14q32.3): deletion I (del G1-EP1-A1-GP-G2-G4) Gm-Am genotype Gm b; -; -A2m2/Gm b ; -; -A2m2. Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). The EZZ deletion (deletion I), which encompasses 160 kb involves two highly homologous regions located 13 kb downstream of IGHG3 and 13 kb downstream of IGHG4.

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures at 5-9x100,000 cells/ml; 5% CO₂; 37°C.

Otras notas

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