KELLY Cell Line human

92110411, human brain, Neuronal

• Sinónimos: NB19 Cells, NB19-RIKEN Cells
• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: KELLY Cell Line human, 92110411, neuroblastoma
• origen biológico: human brain
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Neuronal
• productos: N-myc RNA or protein
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human neuroblastoma

Descripción línea celular

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Aplicación

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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