ND8/34 Cell Line from mouse

92090904, Mouse x Rat Hybridoma nerve, Neuronal

• Código UNSPSC: 41106514

Propiedades

• product name: ND8/34 Cell Line from mouse, 92090904
• origen biológico: Mouse x Rat Hybridoma nerve
• descripción: Mouse neuroblastoma x Rat neurone hybrid
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Neuronal
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse neuroblastoma x Rat neurone hybrid

Descripción línea celular

The hybrid ND8/34 is a subclone of ND8 which was derived after fusion of primary neonatal rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)-derived azaguanine-resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. ND8/34 are electrically excitable to the chemical activator of nociceptive sensory neurons, bradykinin.

Aplicación

Study of neuronal differentiation.

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Otras notas

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