70Z/3

91092002, mouse blood, Lymphoblastoid

• Código UNSPSC: 41106514
• origen biológico: mouse blood
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• productos: Cytoplasmic Immunoglobulin
• receptores: Not specified
• enfermedades relevantes: cancer

Propiedades

• Nombre del producto: 70Z/3, 91092002
• origen biológico: mouse blood
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• productos: Cytoplasmic Immunoglobulin
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse pre-B lymphocyte

Descripción línea celular

Derived from a methyl nitrosourea-induced tumour in a (C57BL/6XDBA/2) F1 mouse. This line is positive for cytoplasmic Ig and negative for surface membrane Ig and TdT.

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 2-5x100,000 cells/ml; 5% CO₂; 37°C.

Otras notas

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr P Kincade, Oklahoma Medical Research Foundation