BLO-11

90112702

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse muscle
• modo de crecimiento: Adherent
• cariotipo: 2n = 40
• morfología: Fibroblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse Moblo/y muscle hereditary emphysema

Descripción línea celular

Established from the abdominal wall muscle of a 30 day old male Blotchy mouse (Mo (blo/y)) with hereditary emphysema. The cells are deficient in lysyl oxidase activity and accumulate high levels of intracellular copper. NOR-10 (Sigma Catalogue number. 90112701) was isolated from the abdominal wall muscle of a normal litter mate of the mouse from which BLO-11 was derived, thus it can serve as a matched control for studies with BLO fibroblasts.

Aplicación

Genetic studies

Medio de cultivo

DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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