CHP 4 (W.W.)

90102537, human skin, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: CHP 4 (W.W.), 90102537
• origen biológico: human skin
• modo de crecimiento: Adherent
• cariotipo: 2n = 46
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human skin asymptomatic Galactosemia

Descripción línea celular

Derived from skin tissue taken from an 8 year old male with asymptomatic galactosemia, despite lacking red cell galactose-1-phosphate uridyl transferase. Sibling of CHP 3 (M.W.) (Sigma catalogue no. 90102536) with the same enzyme deficiency that was symptomatic. Cultures will retain characteristics for at least 30 passages. Ethnicity: Black.

Aplicación

Enzyme deficiency

Medio de cultivo

DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Otras notas

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