BCL1 Clone CW13.20-3B3

90061904, mouse blood, Lymphoblast

• Código UNSPSC: 41106514

Propiedades

• product name: BCL1 Clone CW13.20-3B3, 90061904
• origen biológico: mouse blood
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse BALB/c B cell leukaemia

Descripción línea celular

Derived from BCL1 by limiting dilution and does not spontaneously secrete IgM when cultured in pre-tested batches of FBS. The line is useful for studying soluble factors involved in B-cell differentiation.

Aplicación

B-cell differentiation studies

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells attach lightly to surface which should be tapped sharply to dislodge the cells. Do not allow cells to become confluent, subculture at 75% to 90% confluency; free-floating aggregates acc

Otras notas

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