SAOS-2 Cell Line human

89050205, human bone, Epithelial-like

• Sinónimos: SAOS2 Cells
• Código UNSPSC: 41106514
• origen biológico: human bone
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, (P1) Hyperploid to hypopentaploid
• morfología: Epithelial-like
• productos: Not specified
• receptores: Not specified
• enfermedades relevantes: cancer

Propiedades

• Nombre del producto: SAOS-2 Cell Line human, 89050205, primary osteogenic sarcoma
• origen biológico: human bone
• modo de crecimiento: Adherent
• cariotipo: 2n = 46, (P1) Hyperploid to hypopentaploid
• morfología: Epithelial-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human primary osteogenic sarcoma

Descripción línea celular

Reported to have been derived from an 11 year old female Caucasian. The patient was treated with RTG, methotrexate, adriamycin, vincristine, cytoxan, and aramycin-C. HLA cell line phenotype: A2,3;Bw16,w47.

Aplicación

SAOS-2 cell line has been used to study the importance of cyclin D1 for the activity of lithocholic acid hydroxyamide (LCAHA). It has also been used to study the effect of chromium-enriched diamond-like carbon (DLC) layers on cell adhesion and proliferation.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12,13
D16S539: 12,13
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 18

Medio de cultivo

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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