HL60 Ast.3

88120801, human blood, Lymphoblastoid

• Código UNSPSC: 41106514

Propiedades

• product name: HL60 Ast.3, 88120801
• origen biológico: human blood
• modo de crecimiento: Suspension
• cariotipo: Modal no. = 93, Hypertetraploid
• morfología: Lymphoblastoid
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian promyelocytic leukaemia

Descripción línea celular

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.

Aplicación

Differentiation studies

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic or the cell line will differentiate.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.