RPMI 2650

88031602, human nose, Epithelial

• Sinónimos: RPMI Cell Culture Medium
• Código UNSPSC: 41106514

Propiedades

• product name: RPMI 2650, 88031602
• origen biológico: human nose
• modo de crecimiento: Adherent
• cariotipo: 2n = 46
• morfología: Epithelial
• productos: Mucoid material
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human nasal septum quasidiploid tumour

Descripción línea celular

From the pleural effusion of an extensive malignant tumour of the nasal septum, diagnosed as anaplastic squamous cell carcinoma. Nearly normal karyotype, stability of chromosome number and probable origin from malignant cells make the cell line unique amongst permanent human cell lines. Cells are very small and grow in clusters fusing to form thick layers.

Aplicación

Tumourigenicity studies

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,11
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,11
THO1: 6,8
TPOX: 8
vWA: 16,18

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.