FM3Ats C1.T85

87111904, mouse breast, Lymphoblast

• Código UNSPSC: 41106514

Propiedades

• product name: FM3Ats C1.T85, 87111904
• origen biológico: mouse breast
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse C3H mammary carcinoma

Descripción línea celular

A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (Sigma Catalogue number. 87100804). Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. This abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.

Aplicación

Tumourigenicity studies, chromosome condensation studies.

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 1-3x100,000 cells/ml; 5% CO₂; 33°C.

Otras notas

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