HeLa S3

87110901, human cervix, Epithelial

• Sinónimos: HeLa-S3 Cells, HeLa/S3 Cells, HeLaS3 Cells, S3 HeLa Cells
• Código UNSPSC: 41106514

Propiedades

• product name: HeLa S3, 87110901
• origen biológico: human cervix
• modo de crecimiento: Suspension
• cariotipo: Modal no. 68
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human cervix carcinoma

Descripción línea celular

Derived from the parent HeLa cell line. The cells have been adapted to grow in suspension (spinner) culture and have the same virus susceptibility as the parent line. Very useful in the clonal analysis of mammalian cell populations relating to chromosomal variation, cell nutrition and plaque forming ability. The cells should be handled under laboratory containment level 2.

Aplicación

HeLa S3 has been used to study the cytotoxicity of dinuclear titanium-salan complexes. It has also been used to perform in vitro genotoxicity tests (comet and micronucleus) to study the effects of occupational exposure to carcinogens on sewage workers.

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Medio de cultivo

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Culture in a spinner flask. This cell line may adhere if the culture is not agitated.

Otras notas

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