BB

87101201, fish trunk, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: BB, 87101201
• origen biológico: fish trunk
• modo de crecimiento: Adherent
• cariotipo: 2n = 60
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Fish Brown Bullhead posterior trunk tissue

Descripción línea celular

Derived from the trunk tissue (excluding fins) of a 2 year old Brown Bullhead fish. These cells will tolerate a temperature range of 4-34°C. The optimal temperature range for cell proliferation is 25-30°C. BB is susceptible to infectious pancreatic necrosis virus and channel catfish virus.

Aplicación

Virus studies: Infectious pancreatic necrotis virus and Channel catfish virus and channel catfish virus

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. Note: The cells do not grow well on glass. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 and culture for a

Otras notas

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