NRK-52E

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87012902, rat kidney, Epithelial

• Código UNSPSC: 41106514

Propiedades

• origen biológico: rat kidney
• envase: tube of 5 μg 87012902-DNA-5UG; pkg of vial of cells 87012902-1VL
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat kidney epithelial

Descripción línea celular

Cloned from the same mixed culture of normal rat kidney cells as NRK-49F, but has epithelial-like morphology. It has distinct growth and transforming characteristics distinct from NRK-49F.

Aplicación

NRK-52E has been used to study the effects of altered selenium supply on the expression of endogenous thioredoxin reductase (TrxR).

Medio de cultivo

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5-10% Foetal Bovine Serum (FBS) or New Born Calf Serum (NBCS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Remove all traces of trypsin from cultures during sub-culturing i.e. spin cells or neutralise with serum.

Otras notas

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