GH3

87012603, rat brain, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: GH3, 87012603
• origen biológico: rat brain
• modo de crecimiento: Adherent
• cariotipo: Hyperdiploid, modal no. 60
• morfología: Epithelial
• productos: Growth hormone, prolactin
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat Wistar-Furth pituitary tumour

Descripción línea celular

Derived from a 7 month female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.

Aplicación

Hormone studies

Medio de cultivo

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Upon thawing, the time taken before the first subculture can be performed may be extended (10-15 days). To aid attatchment, flask

Otras notas

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