CRFK (BVD Ag negative)

86093002, feline kidney, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: CRFK (BVD Ag negative), 86093002
• origen biológico: feline kidney
• modo de crecimiento: Adherent
• cariotipo: Hypodiploid, modal no 36
• morfología: Epithelial
• productos: Carcinoembryonic antigen (CEA)
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Cat kidney

Descripción línea celular

CRFK cells were isolated from the cortical portion of the kidneys of a 10-12 week old normal female domestic cat. CRFK are extensively used in feline virus research. Susceptible to herpesvirus and feline picornaviruses producing CPE. BVD Ag negative.

Aplicación

Crandell feline kidney cells (CRFK) (BVD Ag negative) have been used in viral studies.

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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