Detroit 551

86082802, human skin (embryonic), Fibroblast

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: Detroit 551, 86082802
• origen biológico: human skin (embryonic)
• envase: tube of 5 μg 86082802-DNA-5UG; pkg of vial of cells 86082802-1VL
• modo de crecimiento: Adherent
• cariotipo: 2n = 46
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian embryo skin

Descripción línea celular

Derived from a skin biopsy of a normal Caucasian female embryo. Cells may be considered normal and may serve as controls for study of cells having mutant chromosomes. Cells support growth of herpes viruses and SV40 virus can induce transformation.

Aplicación

Chromosome and virus studies (Normal control): herpesviruses, SV40

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan; at most 25 subcultures from original tissue.

Otras notas

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