A7r5 Cell Line from rat

86050803, rat muscle, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: A7r5 Cell Line from rat, 86050803
• origen biológico: rat muscle
• descripción: Rat BDIX smooth muscle embryonic aorta
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Muscle-type isoenzymes
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat BDIX smooth muscle embryonic aorta

Descripción línea celular

Flat ribbon-like cells differentiating to parallel arrays of spindle shaped cells. Derived from the thoracic aorta of an embryonic BDIX rat. As cells achieve the stationary phase there is an increase in myokinase and creatine phosphokinase (CPK) activity. After cessation of cell division a muscle type CPK isoenzyme is synthesised.

Aplicación

A7r5 Cell Line from rat has been used to study the effect of angiotensin II on vascular oxidative stress. It has been used in PLA2 inhibitory study to understand the role of PLA2 on formation of lipid droplets.

Biochemical and biophysical studies

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. After resuscitation cells may take up to 7 days before a monolayer forms and growth can be very slow in general. Cells also grow in clumps.

Otras notas

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