MiCL1 (S+L-)

86020603, mink lung, Fibroblast-like

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: MiCL1 (S+L-), 86020603
• origen biológico: mink lung
• envase: tube of 5 μg 86020603-DNA-5UG; pkg of vial of cells 86020603-1VL
• modo de crecimiento: Adherent
• cariotipo: 2n = 30
• morfología: Fibroblast-like
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mink lung, Moloney murine sarcoma virus infected

Descripción línea celular

A clone of the mink lung cell line Mv.1.Lu (Sigma Catalogue number. 88050503). Isolated from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.

Aplicación

Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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