H6/31HL

85102214, Reactivity: Anti-IgD, mouse

• Código UNSPSC: 41106514

Propiedades

• origen biológico: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Mieloma: P3X63Ag8
• Reactividad: Anti-IgD, mouse
• técnicas: cell culture | mammalian: suitable
• isotipo: IgM
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse P3X63Ag8 x Mouse BALB/c spleen

Descripción línea celular

The antibody is directed against ′IgD-like′ molecules of mice with IgB haplotype. The target antigen has a molecular weight of 185kDa and is found on a high proportion of mature lymphoid cells, particularly on cells from Peyer′s patches. It is not detected on thymus cells.

Inmunógeno

Two grafts of full thickness skin from B10 mice followed by B10 spleen cells

Medio de cultivo

DMEM + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.