Do-11-10

85082301, mouse unknown/unspecified, Not specified

• Código UNSPSC: 41106514

Propiedades

• product name: Do-11-10, from mouse unknown/unspecified, 85082301
• origen biológico: mouse unknown/unspecified
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147

Descripción línea celular

Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.

Medio de cultivo

DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.

Otras notas

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