3T3 Swiss Albino

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85022108, mouse embryo, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse embryo
• envase: tube of 5 μg 85022108-DNA-5UG; pkg of vial of cells 85022108-1VL
• modo de crecimiento: Adherent
• cariotipo: 2n = 40; hypertriploid
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

Product has lost contact inhibition

Origen línea celular

Mouse Swiss Albino embryo fibroblast

Descripción línea celular

Evolved under conditions favouring retention of the property of contact inhibition of cell proliferation; they exhibit much more contact inhibition than diploid fibroblasts freshly plated in culture. Readily transformed by polyoma and SV40 viruses resulting in reduced sensitivity to contact inhibition. Cells have now lost their contact inhibition.

Aplicación

Contact inhibition and virus studies: polyoma and SV40 viruses

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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