A2H

85011441, hamster ovary, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: A2H, 85011441
• origen biológico: hamster ovary
• envase: tube of 5 μg 85011441-DNA-5UG; pkg of vial of cells 85011441-1VL
• modo de crecimiento: Adherent
• cariotipo: 2n = 22
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Hamster Chinese ovary

Descripción línea celular

Used for the induction and isolation of nutritionally deficient mutants. A reclone of ′Clone A′ established by Puck (the ′H′ denotes MRC Harwell). Useful for genetic studies due to the low chromosome number.

Aplicación

Genetic studies

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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