BHK 21 (Clone 13) from hamster

85011433, from hamster kidney

• Sinónimos: BHK-21 (C-13) Cells, BHK-21 C-13 Cells, BHK21 clone 13 Cells, BHK21-C13 Cells, BHK21/C13 Cells
• Código UNSPSC: 41106514

Propiedades

• origen biológico: hamster kidney
• modo de crecimiento: Adherent
• cariotipo: 2n = 44, pseudodiploid
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Hamster Syrian kidney

Descripción línea celular

Subclone of parent line derived from 5 1-day-old unsexed hamster kidneys. Used extensively for virus replication studies i.e. poliovirus, rabies, foot and mouth disease, VSV (Indiana strain), herpes simplex, Ad25 and arboviruses.

Aplicación

BHK 21 (Clone 13) from hamster has been used in viral transfection studies.

Virus replication studies

Medio de cultivo

GMEM + 2mM Glutamine + 5% Tryptose Phosphate Broth + 5-10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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