MRC-5 SV2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 84100401, human lung (fetal), Fibroblast

• Sinónimos: MRC-5V2 Cells, MRC5V2 Cells
• Código UNSPSC: 41106514

Propiedades

• origen biológico: human lung (fetal)
• envase: tube of 5 μg 84100401-DNA-5UG; pkg of vial of cells 84100401-1VL
• modo de crecimiento: Adherent
• cariotipo: Modal no. = 60-80, hyperdiploid
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human foetal lung, SV40 transformed

Descripción línea celular

Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.

Aplicación

Transformation and virus studies: SV40

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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