KARPAS 1718

08072401, human spleen (lymphoma), Single round to polymorphic cells growing in suspension

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: KARPAS 1718, 08072401
• origen biológico: human spleen (lymphoma)
• envase: tube of 5 μg 08072401-DNA-5UG; pkg of vial of cells 08072401-1VL
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Single round to polymorphic cells growing in suspension
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human splenic lymphoma

Descripción línea celular

KARPAS 1718 is derived from the peripheral blood of a patient diagnosed with transformed splenic lymphoma with villous lymphocytes

Aplicación

Oncology research

Perfil del ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 8,13
D16S539: 11,13
D5S818: 11,12
D7S820: 8
THO1: 6
TPOX: 8,11
vWA: 14

Medio de cultivo

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵ cells/ml. Viability may be poor on resuscitation and may decrease further within the first 48 hours, the culture may appear non-viable at this time. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 105 cells/ml, 5% CO₂, 37°c. For cryopreservation of KARPAS 1718, freeze at approximately 2 x 107 cells/ml in 90% FBS + 10% DMSO.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.