N1E-115-1

08062511

• Código UNSPSC: 41106514

Propiedades

• origen biológico: mouse nerve
• modo de crecimiento: Adherent
• cariotipo: modal no. of chromosomes is 192 see reference
• morfología: neuronal
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse neuroblastoma

Descripción línea celular

A subclone of the cell line N1E-115 established by Amano et al. The cell line N1E-115 is an adrenergic cell line derived from mouse neuroblastoma C1300. (Mouse strain A/Jax). N1E-115 cells are reported to be slightly contaminated with with a cell type of the morphology of C6 glioma cells. It is recommended to use N1E-115-1, a subclone of N1E-115, that is devoid of the contamination.

Aplicación

can be used as a neuronal model

Medio de cultivo

DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Population doubling approximately 20 hours.

Otras notas

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